Transgenic expression and purification of myosin isoforms using the Drosophila melanogaster indirect flight muscle system
نویسندگان
چکیده
منابع مشابه
The development of indirect flight muscle innervation in Drosophila melanogaster
We have examined the development of innervation to the indirect flight muscles of Drosophila. During metamorphosis, the larval intersegmental nerve of the mesothorax is remodelled to innervate the dorsal longitudinal muscles and two of the dorsoventral muscles. Another modified larval nerve innervates the remaining dorsoventral muscle. The dorsal longitudinal muscles develop using modified larv...
متن کاملAn embryonic myosin converter domain influences Drosophila indirect flight muscle stretch activation, power generation and flight.
Stretch activation (SA) is critical to the flight ability of insects powered by asynchronous, indirect flight muscles (IFMs). An essential muscle protein component for SA and power generation is myosin. Which structural domains of myosin are significant for setting SA properties and power generation levels is poorly understood. We made use of the transgenic techniques and unique single muscle m...
متن کاملMutations affecting the indirect flight muscles of Drosophila melanogaster.
The development of the indirect flight muscles of Drosophila melanogaster was studied by analysing mutations that cause flightlessness. Twenty-five mutations on the X-chromosome and two on the third chromosome were examined. The X-chromosomal mutations form ten complementation units. The ten loci were assigned preliminary map positions by meiotic recombination and deficiencies and duplications....
متن کاملParamyosin phosphorylation site disruption affects indirect flight muscle stiffness and power generation in Drosophila melanogaster.
The phosphoprotein paramyosin is a major structural component of invertebrate muscle thick filaments. To investigate the importance of paramyosin phosphorylation, we produced transgenic Drosophila melanogaster in which one, three, or four phosphorylatable serine residues in the N-terminal nonhelical domain were replaced by alanines. Depending on the residues mutated, transgenic lines were eithe...
متن کاملIn vivo x-ray diffraction of indirect flight muscle from Drosophila melanogaster.
Small-angle x-ray diffraction from isolated muscle preparations is commonly used to obtain time-resolved structural information during contraction. We extended this technique to the thoracic flight muscles of living fruit flies, at rest and during tethered flight. Precise measurements at 1-ms time resolution indicate that the myofilament lattice spacing does not change significantly during osci...
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ژورنال
عنوان ژورنال: Methods
سال: 2012
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2011.12.002